Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EZH2

Cell type

Cell type Class
Blood
Cell type
OCI-LY1
NA
NA

Attributes by original data submitter

Sample

source_name
OCI-Ly1 cell line
cell type
OCI-Ly1 cell line
treatment
NA
chip antibody
anti-EZH2 (Millipore, 07-689, lot DAM1834941)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed as previously described (Ci et al., Blood, 2009). Briefly, 108 cells were fixed with 1% formaldehyde, lysed, and sonicated (Branson Sonicator; Branson) leading to a DNA average size of 200 bp. Five µg of antibodies were added to the precleared sample and incubated overnight at 4°C. The complexes were purified using protein-A beads (Roche) followed by elution from the beads and decrosslinking. DNA was purified using PCR purification columns (QIAGEN). ChIP-seq and RNA-seq libraries were prepared using the Illumina ChIP-Seq and TruSeq RNA sample kits, respectively, according to the manufacturer. Libraries were validated using the Agilent Technologies 2100 Bioanalyzer and Quant-iT™ dsDNA HS Assay (Life Technologies)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
92598605
Reads aligned (%)
66.2
Duplicates removed (%)
29.5
Number of peaks
1925 (qval < 1E-05)

hg38

Number of total reads
92598605
Reads aligned (%)
68.6
Duplicates removed (%)
27.6
Number of peaks
2395 (qval < 1E-05)

Base call quality data from DBCLS SRA