GSM2564966: KO H4K16 Ab Rep2 ChIP seq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H4K16ac
Cell type
Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast
Attributes by original data submitter
Sample
source_name
Mouse Embryonic Fbroblast
cell type
Mouse Embryonic Fbroblast (MEF)
genotype
Peg3-KO
passages
3 to 5
strain
C57BL/6
chip antibody
H4K16ac (Millipore Cat No 07329)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
MEFs were first cross-linked with 1% formaldehyde for 20 mins, and then lysed with the buffer containing protease inhibitor cocktail (Millipore, Cat. No. 539131). The released nuclei were fractionated with sonication to a pool of DNA fragments size-ranging from 300 to 1,000 bp in length. The prepared chromatin was immunoprecipitated with the H4K16ac antibody. The two pools of the immunoprecipitated DNA along with the two corresponding input DNA were used for constructing libraries for ChIP-seq experiments according to the manufacturers’ protocol (Illumina FC4014003).