Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Muscle

Cell type

Myoblasts

MeSH Description

Embryonic (precursor) cells of the myogenic lineage that develop from the MESODERM. They undergo proliferation, migrate to their various sites, and then differentiate into the appropriate form of myocytes (MYOCYTES, SKELETAL; MYOCYTES, CARDIAC; MYOCYTES, SMOOTH MUSCLE).

Sample

source_name

skeletal muscle

strain

C57BL/6

cell type

primary myoblasts

age

isolated from 10-day-old mice

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Primary myoblasts (PriMBs) were isolated from limb muscle of 10-day old mice using the preplating technique and cultivated to about 70% confluency. Chromatin was prepared and ChIP performed according to Arrigoni, L. et al. (2016) Nucleic Acids Res. 55, e67. Libraries were prepared using the NEBNext Ultra DNA library preparation kit (NEB, E7370S). Adapter-ligated fragments were amplified with 12 PCR cycles and sequenced on the Illumina HiSeq 2500 platform.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

52591193

Reads aligned (%)

95.9

Duplicates removed (%)

10.6

Number of peaks

249 (qval < 1E-05)

mm9

Number of total reads

52591193

Reads aligned (%)

95.7

Duplicates removed (%)

11.3

Number of peaks

255 (qval < 1E-05)