Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Others
Cell type
Pleomorphic sarcoma
NA
NA

Attributes by original data submitter

Sample

source_name
UPS_H3K27Ac
cell type
Undifferentiated Pleomorphic Sarcoma (UPS)
chip antibody
H3K27Ac; Abcam ab4729

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Samples were homogenized with rotor-stator device. Lysates were sonicated via Bioruptor. Protein-DNA complexes were isolated via immunoprecipitation and prepared for sequencing with Rubicon Thruplex DNA-seq kit. ChIP-seq via single-end 75-bp sequencing on Nextseq 500.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
65147619
Reads aligned (%)
98.1
Duplicates removed (%)
7.9
Number of peaks
31002 (qval < 1E-05)

hg19

Number of total reads
65147619
Reads aligned (%)
97.5
Duplicates removed (%)
8.8
Number of peaks
30854 (qval < 1E-05)

Base call quality data from DBCLS SRA