Antigen

Antigen Class

TFs and others

Antigen

Trp63

Cell type

Cell type Class

Epidermis

Cell type

Keratinocytes

MeSH Description

Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.

Sample

source_name

cultured keratinocytes cell

cell type

cultured keratinocytes

genotype

Wild type

chip antibody

P63 antibody, Cell Signaling, #4892

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Back skin is dissected and treated with Collagenase and Trypsin to achieve single cell suspension. Epidermal cells isolated by FACS of GFP positive cells. Dermal cells isolated by FACS through CD140a antibody staining. SMARTer Directional cDNA Library Construction Kit and NEBNext® Ultra™ Directional RNA Library Prep Kit ; ATAC-seq, protocol as described in Buenrostro et al., Nat Methods, 2013; ChiP-seq, protocol described in Wang et al., RNA, 2013.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

8828809

Reads aligned (%)

89.9

Duplicates removed (%)

65.9

Number of peaks

962 (qval < 1E-05)

mm9

Number of total reads

8828809

Reads aligned (%)

89.9

Duplicates removed (%)

65.9

Number of peaks

951 (qval < 1E-05)