Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HMBOX1

Cell type

Cell type Class
Lung
Cell type
WI-38
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
WI-38 VA13 ALT (+) cells, HMBOX1 ChIP
cell line
WI-38
alt status
+
chip antibody
anti-HMBOX1 / Rabbit polyclonal IgG (Abcam ab97643)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared by Fasteris SA (Switzerland) and were sequenced on HiSeq 2000.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
27643358
Reads aligned (%)
63.9
Duplicates removed (%)
5.8
Number of peaks
1433 (qval < 1E-05)

hg38

Number of total reads
27643358
Reads aligned (%)
65.5
Duplicates removed (%)
4.4
Number of peaks
1478 (qval < 1E-05)

Base call quality data from DBCLS SRA