Antigen

Antigen Class

TFs and others

Antigen

HMBOX1

Cell type

Cell type Class

Lung

Cell type

WI-38

Primary Tissue

Lung

Tissue Diagnosis

Normal

Sample

source_name

WI-38 VA13 ALT (+) cells, HMBOX1 ChIP

cell line

WI-38

alt status

+

chip antibody

anti-HMBOX1 / Rabbit polyclonal IgG (Abcam ab97643)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared by Fasteris SA (Switzerland) and were sequenced on HiSeq 2000.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

27643358

Reads aligned (%)

68.5

Duplicates removed (%)

4.4

Number of peaks

1478 (qval < 1E-05)

hg19

Number of total reads

27643358

Reads aligned (%)

67.9

Duplicates removed (%)

5.8

Number of peaks

1433 (qval < 1E-05)