SRX268247
GSM1123982: ER ChIP-Seq (Dex + E2) r1; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Esr1

Cell type

Cell type Class
Breast
Cell type
7438
NA
NA

Attributes by original data submitter

Sample

source_name
7438 cells
cell type
mouse mammary cells (express GFP-GR and Cherry-ER under a tet regulated promoter)
treatment
Dexamethasone + Estradiol for 30 minutes
cell line
7438 cells
replicate
replicate 1
chip antibodies
cocktail of Ab-10 from Labvision and sc-543 from Santa Cruz

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and GR-DNA complexes or ER-DNA complexes were isolated with antibody

Sequencing Platform

instrument_model
Illumina Genome Analyzer

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
22831599
Reads aligned (%)
95.4
Duplicates removed (%)
25.1
Number of peaks
9081 (qval < 1E-05)

mm9

Number of total reads
22831599
Reads aligned (%)
95.2
Duplicates removed (%)
25.2
Number of peaks
9143 (qval < 1E-05)

Base call quality data from DBCLS SRA