SRX268236
GSM1123971: GR ChIP-Seq (+Dex) r2; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Nr3c1

Cell type

Cell type Class
Breast
Cell type
7438
NA
NA

Attributes by original data submitter

Sample

source_name
7438 cells
cell type
mouse mammary cells (express GFP-GR and Cherry-ER under a tet regulated promoter)
treatment
Dexamethasone for 30 minutes
cell line
7438 cells
replicate
replicate 2
chip antibodies
cocktail of PA-510A and PA-511A from Affinity BioReagents and sc-1004 from Santa Cruz

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and GR-DNA complexes or ER-DNA complexes were isolated with antibody

Sequencing Platform

instrument_model
Illumina Genome Analyzer

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
32153805
Reads aligned (%)
95.4
Duplicates removed (%)
34.1
Number of peaks
16018 (qval < 1E-05)

mm9

Number of total reads
32153805
Reads aligned (%)
95.2
Duplicates removed (%)
34.2
Number of peaks
16041 (qval < 1E-05)

Base call quality data from DBCLS SRA