GSM1125036: Input Control ChIPSeq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Adipocyte
Cell type
White adipocytes
NA
NA
Attributes by original data submitter
Sample
source_name
iWAT-derived adipocytes
antibody
none (input)
gender
male
animal model
NMRI
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
eWAT-, iWAT-, and BAT-derived adipocytes were cross-linked using 0.5 M disuccinimidyl glutarate (DSG) (Proteochem, Denver, CO) for 45 min followed by cross-linking using 1% formaldehyde for 10 min. Cross-linking was stopped by the addition of glycine to a final concentration of 0.125 M for 10 min, followed by addition of ChIP lysis buffer (1% Triton X-100, 0.1% SDS, 150 mM NaCl, 1 mM EDTA, and 20 mM Tris, pH 8.0). Samples were sonicated using the Diagenode Bioruptor twin (3 x14 cycles, 30 s on/off, maximum level). Chromatin IP was performed using anti-PPARγ (sc-7196; Santa Cruz). ChIPed DNA was sequenced according to the manufacturer’s instructions (Illumina).