Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Pancreas

Cell type

Pancreatic cancer

NA

NA

Sample

source_name

Pancreatic Cancer Cells

sample type

adenocarcinoma

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

dna extraction by qiagen ATAC-seq was performed as per protocol established by Buenrostro JD et al, ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide. Curr Protoc Mol Biol 109: 21 29 21-29. Used KAPA LT DNA prep kit and generated PCR Free libraries using 500 ng gDNA.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

236411701

Reads aligned (%)

98.6

Duplicates removed (%)

22.5

Number of peaks

68185 (qval < 1E-05)

hg19

Number of total reads

236411701

Reads aligned (%)

98.4

Duplicates removed (%)

22.5

Number of peaks

67280 (qval < 1E-05)