Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Pancreas

Cell type

Pancreas

MeSH Description

A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.

Sample

source_name

Pancreatic control cells

sample type

Control

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

dna extraction by qiagen ATAC-seq was performed as per protocol established by Buenrostro JD et al, ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide. Curr Protoc Mol Biol 109: 21 29 21-29. Used KAPA LT DNA prep kit and generated PCR Free libraries using 500 ng gDNA.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

84956277

Reads aligned (%)

98.6

Duplicates removed (%)

43.6

Number of peaks

57819 (qval < 1E-05)

hg19

Number of total reads

84956277

Reads aligned (%)

98.3

Duplicates removed (%)

43.7

Number of peaks

57468 (qval < 1E-05)