Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Larvae

Cell type

3rd instar

NA

NA

Sample

source_name

Whole 3rd Instar Larvae

developmental stage

3rd instar larvae

tissue

Whole larvae

Sex

mixed

genotype

HWT

target molecule

genomic DNA

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Nuclei were isolated from whole larvae and incubated with Tn5 transposase from Nextera DNA Library Prep Kit (Illumina) according to manufacturer's protocols and standard ATAC-seq protocol (Buenrostro et al. 2013) Libraries were prepared according to Illumina Nextera DNA Library Prep Kit protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

22963239

Reads aligned (%)

92.2

Duplicates removed (%)

45.1

Number of peaks

19962 (qval < 1E-05)

dm3

Number of total reads

22963239

Reads aligned (%)

92.3

Duplicates removed (%)

44.5

Number of peaks

19464 (qval < 1E-05)