Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

OS

cell line

U20S AsiSI

cell type

osteosarcoma

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Nascent RNA was isolated using immunopurification of mammalian nascent elongating transcripts (mNET-IP) as described in Nojima 2016. For ChIP-seq: Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with Tyrosine 1-p antibody.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

48219260

Reads aligned (%)

77.3

Duplicates removed (%)

90.4

Number of peaks

225 (qval < 1E-05)

hg19

Number of total reads

48219260

Reads aligned (%)

76.7

Duplicates removed (%)

90.7

Number of peaks

154 (qval < 1E-05)