GSM2544915: hos1 aid 0; Saccharomyces cerevisiae; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Unclassified
Cell type
Unclassified
NA
NA
Attributes by original data submitter
Sample
source_name
yeast cells
genotype
Hos1 depletion
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For crosslinking, cells were incubated with 1% formaldehyde overnight at 4°C. Cells were harvested and washed three times with ice-cold TBS, and cells were transferred to 1.5 ml tube. For cell breakage, 300 ml of lysis buffer and glass beads were added to the tube, and placed tubes into the cells disruptor (FastPrep-24 5G), and disrupt cell wall at speed 6.0 m/sec for 40 sec, repeat the disruption procedure once more to achieve better results. The cell lysate was sonicated with Bioruptor (Diagenode, 30sec ´ 30 cycles with 30 sec intervals with high intensity), resulting in an average fragment size of 300~200 bp. The lysate was clarified by centrifugation at 13,000 rpm for 5 minutes. For immunoprecipitation, 60 ml of magnetic beads (Dynabeads Pan Mouse IgG, Invitrogen) were added following pre-incubatition with an anti-HA monoclonal antibody (HA.11, Covance).