Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Lung

Cell type

Non-small cell lung cancer

NA

NA

Sample

source_name

Non-small cell lung cancer cells

drugs treated

DMSO

chip-antibody

H3K27 acetylation (Abcam, ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using Rubicon Genomics Thruplex-DNaseq sample preparation kits from 2 ng immunoprecipitated DNA according to manufacturer’s protocol. The finished double strand DNA (dsDNA) libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer’s protocols. Uniquely indexed libraries were pooled in equimolar ratios and sequenced on an Illumina NextSeq500 with single-end 75bp reads by the Dana-Farber Cancer Institute Molecular Biology Core Facilities.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

89720273

Reads aligned (%)

89.3

Duplicates removed (%)

5.4

Number of peaks

2534 (qval < 1E-05)

hg19

Number of total reads

89720273

Reads aligned (%)

88.5

Duplicates removed (%)

6.9

Number of peaks

1514 (qval < 1E-05)