ChIP-Atlas: SRX2652391

Antigen

library_strategy: ATAC-seq
library_source: GENOMIC
library_selection: other
library_construction_protocol: All cells described here were FACS sorted prior to performing the assay. One vial of 15,000 frozen cells in 100 μl freezing medium was thawed at 37˚C. 70 μl for bulk ATAC-seq were then added to 500 μl of warm IMDM with 10% FBS. For bulk ATAC-seq, the cells were split into 2 tubes of 5,000 cells and used as technical replicates. Cells were washed twice in 1x PBS, all supernatant was carefully removed without disturbing the cell pellet, and cells were resuspended in 40 μl of transposition mix (20 μl of 2x TD buffer, 2 μl of TDE1, 0.2 μl of 2% digitonin, 13.33 μl of 1x PBS, and 4.47 μl of nuclease-free water) (Illumina, FC-121-1030; Promega, G9441), here the transposition reactions were scaled down to compensate for cell loss during washes. Transposition reactions were incubated at 37 ˚C for 30 min in an Eppendorf ThermoMixer with agitation at 300 rpm. The transposed DNA fragments were purified and amplified as described.