GSM2543091: BM0828-MEGA1-A-151109; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
CD34+
NA
NA
Attributes by original data submitter
Sample
source_name
Bone Marrow CD34+
cell type
Mega
tissue
CD34+ Bone Marrow
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
All cells described here were FACS sorted prior to performing the assay. One vial of 15,000 frozen cells in 100 μl freezing medium was thawed at 37˚C. 70 μl for bulk ATAC-seq were then added to 500 μl of warm IMDM with 10% FBS. For bulk ATAC-seq, the cells were split into 2 tubes of 5,000 cells and used as technical replicates. Cells were washed twice in 1x PBS, all supernatant was carefully removed without disturbing the cell pellet, and cells were resuspended in 40 μl of transposition mix (20 μl of 2x TD buffer, 2 μl of TDE1, 0.2 μl of 2% digitonin, 13.33 μl of 1x PBS, and 4.47 μl of nuclease-free water) (Illumina, FC-121-1030; Promega, G9441), here the transposition reactions were scaled down to compensate for cell loss during washes. Transposition reactions were incubated at 37 ˚C for 30 min in an Eppendorf ThermoMixer with agitation at 300 rpm. The transposed DNA fragments were purified and amplified as described.