GSM2537644: ZHBTc4 Dox minus H3v3 lot2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3.3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
Embryonic stem cells
cell type
Embryonic stem cells
passages
10-20
strain
129/Ola
antibody
H3.3 (rat monoclonal antibody using in Harada,A et al, The EMBO Journal, 2012.)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) according to manufacturer's instructions.