Antigen

Antigen Class

Histone

Antigen

H3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

Embryonic stem cells

cell type

Embryonic stem cells

passages

10-20

strain

129/Ola

antibody

H3 (Abcam, ab1791)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) according to manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 1000

mm10

Number of total reads

22282232

Reads aligned (%)

97.6

Duplicates removed (%)

11.9

Number of peaks

409 (qval < 1E-05)

mm9

Number of total reads

22282232

Reads aligned (%)

97.4

Duplicates removed (%)

12.0

Number of peaks

400 (qval < 1E-05)