H3K27me3 (mouse monoclonal antibody provided by Dr. H. Kimura using in Kimura H et al., Cell Struct. Funct., 2008.)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) according to manufacturer's instructions.