H3K4me3 (mouse monoclonal antibody provided by Dr. H. Kimura using in Hayashi-Takanaka,Y et al., Nucleic Acids Research, 2011. )
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) according to manufacturer's instructions.