Antigen

Antigen Class

Histone

Antigen

H3.3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

Embryonic stem cells

cell type

Embryonic stem cells

passages

10-20

strain

129/Ola

antibody

H3.3 (rat monoclonal antibody using in Harada,A et al, The EMBO Journal, 2012.)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) according to manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 1000

mm10

Number of total reads

28087914

Reads aligned (%)

97.6

Duplicates removed (%)

12.9

Number of peaks

568 (qval < 1E-05)

mm9

Number of total reads

28087914

Reads aligned (%)

97.2

Duplicates removed (%)

12.8

Number of peaks

574 (qval < 1E-05)