Antigen

Antigen Class

Bisulfite-Seq

Antigen

Bisulfite-Seq

Cell type

Cell type Class

Neural

Cell type

Anterior cingulate cortex

NA

NA

Sample

source_name

NeuN positive nuclei sorted from anterior cingulate cortex (BA24)

cell type

NeuN pos

tissue

anterior cingulate cortex (BA24)

individual id

5248

Sequenced DNA Library

library_strategy

Bisulfite-Seq

library_source

GENOMIC

library_selection

RANDOM

library_construction_protocol

Genomic DNA was extracted using the Epicentre MasterPure DNA Purification Kit (cat#: MCD85201) NEBNext Ultra DNA library Prep kit for Illumina followed by size selection (300-400bp), bisulfite conversion with EZ DNA Methylation-Gold kit (cat#:D5005,Zymo), and amplification using Kapa hifi Uracil+ (cat#:KK282, Kapa Biosystems).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

226409362

Reads aligned (%)

90.0

Coverage rate (×)

5.3

Number of hyper MRs

221597 (qval < 1E-05)

hg19

Number of total reads

226409362

Reads aligned (%)

90.1

Coverage rate (×)

5.9

Number of hyper MRs

206335 (qval < 1E-05)