anti-Polymerase II (Clone: N20; Vendor: Santa Cruz Biotechnology, catalogue number: SC-899 X, type: rabbit polyclonal IgG)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin IP was performed using anti-Polymerase II (Pol II N-20 (Santa Cruz sc-899) ) antibody. Precipitated genomic DNA or whole genomic DNA was used for library preparation. Library preparation was done using Kapa Library Preparation Kit (Roche). Barcoded samples were multiplexed and sequenced 50bp SE on a HighSeq2500 (Illumina).