Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

Bone Marrow Cells

MeSH Description

Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.

Sample

source_name

bone marrow

tissue

bone marrow

culture

primary cells (short term)

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Cells were collected, seperated from feeders by pre-plating and genomic DNA was tagmented using the Tn5 transposase. Libraries were constructed using the Illumina Nextera DNA Library Prep Kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

91863341

Reads aligned (%)

95.6

Duplicates removed (%)

47.3

Number of peaks

34714 (qval < 1E-05)

mm9

Number of total reads

91863341

Reads aligned (%)

95.5

Duplicates removed (%)

47.4

Number of peaks

36746 (qval < 1E-05)