Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Neural

Cell type

Astrocytes

MeSH Description

A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.

Sample

source_name

H3K27ac_Control_eA

strain background

C57/BL-6

cell type

early astrocytes (eA)

chip antibody

Anti-Histone H3 (acetyl K27) antibody (Abcam ab4729)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For RNA-seq, RNA was harvested using Trizol reagent. For ChIP-seq, lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody against H3K27ac and H3K4me1 histone marks. Libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

20670032

Reads aligned (%)

97.3

Duplicates removed (%)

8.6

Number of peaks

29301 (qval < 1E-05)

mm9

Number of total reads

20670032

Reads aligned (%)

97.1

Duplicates removed (%)

8.6

Number of peaks

29291 (qval < 1E-05)