Antigen

Antigen Class

Histone

Antigen

H3K36me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H9

NA

NA

Sample

dev stage

embryonic

cell type

H9

sex

female

sample type

stem cell

donor ID

ENCDO222AAA

age

5 day

health state

healthy

lab

Bradley Bernstein, Broad

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

general protocol: https://www.encodeproject.org/documents/be2a0f12-af38-430c-8f2d-57953baab5f5/@@download/attachment/Epigenomics_Alternative_Mag_Bead_ChIP_Protocol_v1.1_exp.pdf

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

44252675

Reads aligned (%)

94.5

Duplicates removed (%)

6.5

Number of peaks

1635 (qval < 1E-05)

hg19

Number of total reads

44252675

Reads aligned (%)

93.3

Duplicates removed (%)

8.3

Number of peaks

1196 (qval < 1E-05)