Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Bone

Cell type

A-673

Primary Tissue

Skeletal Muscle

Tissue Diagnosis

Rhabdomyosarcoma

Sample

dev stage

unknown

cell line

A673

sex

female

sample type

immortalized cell line

donor ID

ENCDO027VXA

age

15 year

lab

Bradley Bernstein, Broad

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

general protocol: https://www.encodeproject.org/documents/be2a0f12-af38-430c-8f2d-57953baab5f5/@@download/attachment/Epigenomics_Alternative_Mag_Bead_ChIP_Protocol_v1.1_exp.pdf

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

19046661

Reads aligned (%)

98.1

Duplicates removed (%)

7.9

Number of peaks

892 (qval < 1E-05)

hg19

Number of total reads

19046661

Reads aligned (%)

97.6

Duplicates removed (%)

8.0

Number of peaks

828 (qval < 1E-05)