Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Digestive tract

Cell type

SW 48

Primary Tissue

Colon

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

colon cancer cells

cell line

SW48

antibody

H3K27ac

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were cross-linked in 1% formalin and were sonicated in SDS-lysis buffer. Chromatin was precipitated in ChIP dilution buffer using an anti-H3K27ac antibody. DNA was purified by phenol and chloroform extraction and ethanol precipitation. Libraries were prepared according to Illumina's instructions. Libraries were sequenced using Illumina HiSeq4000.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

22284262

Reads aligned (%)

98.9

Duplicates removed (%)

13.5

Number of peaks

23407 (qval < 1E-05)

hg19

Number of total reads

22284262

Reads aligned (%)

98.6

Duplicates removed (%)

14.1

Number of peaks

23485 (qval < 1E-05)