GSM2532773: Caco2 H3K27ac ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Digestive tract
Cell type
Caco-2
Primary Tissue
Colon
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
colon cancer cells
cell line
caco-2
antibody
H3K27ac
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked in 1% formalin and were sonicated in SDS-lysis buffer. Chromatin was precipitated in ChIP dilution buffer using an anti-H3K27ac antibody. DNA was purified by phenol and chloroform extraction and ethanol precipitation. Libraries were prepared according to Illumina's instructions. Libraries were sequenced using Illumina HiSeq4000.