Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Epidermis

Cell type

Keratinocytes

MeSH Description

Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.

Sample

dev stage

unknown

cell type

keratinocyte

sex

female

sample type

primary cell

donor ID

ENCDO268AAA

age

unknown

health state

unknown

lab

Bradley Bernstein, Broad

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

extraction method: see document general protocol: https://www.encodeproject.org/documents/f10bdd36-e370-4777-a7f3-83091c115749/@@download/attachment/wgEncodeBroadHistone.html.pdf

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

21067457

Reads aligned (%)

53.8

Duplicates removed (%)

2.8

Number of peaks

574 (qval < 1E-05)

hg19

Number of total reads

21067457

Reads aligned (%)

53.1

Duplicates removed (%)

3.8

Number of peaks

737 (qval < 1E-05)