Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Muscle

Cell type

Muscle, Smooth

MeSH Description

Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed)

Sample

age

5 day

donor ID

ENCDO222AAA

dev stage

embryonic

sample type

in vitro differentiated cells

cell type

smooth muscle cell

sex

female

health state

healthy

lab

Richard Myers, HAIB

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

general protocol: https://www.encodeproject.org/documents/be2a0f12-af38-430c-8f2d-57953baab5f5/@@download/attachment/Epigenomics_Alternative_Mag_Bead_ChIP_Protocol_v1.1_exp.pdf

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

46655451

Reads aligned (%)

96.7

Duplicates removed (%)

4.5

Number of peaks

975 (qval < 1E-05)

hg19

Number of total reads

46655451

Reads aligned (%)

95.9

Duplicates removed (%)

5.4

Number of peaks

835 (qval < 1E-05)