GSM2523110: ChIP DNMT3B Ctrl1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
TFs and others
Cell type Class
B Lymphoblastoid cell line
Attributes by original data submitter
Sequenced DNA Library
Lysates were clarified from sonicated nuclei. Histone-DNA and DNMT3B-DNA complexes were isolated with specific antibodies. Immunoprecipitated complexes were recovered with protein A sepharose (Pharmacia), washed with low and high salt buffers, reverse-crosslinked, and purified. DNMT3B and H3K36me3 libraries were prepared according to Illumina's instructions accompanying the Nextera DNA Sample Kit. H3K27me3 and H3K4me3 libraries were prepared according to SOLiD protocol (Applied Biosystem).