GSM2520030: IP K79Me3 KD Ino80; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K79me3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
ESC cells
cell type
ESC cells
antibody
Anti H3K79Me3 antibody from Abcam (ab8898)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, cells were harvested and crosslinked in suspension with 1% formaldehyde for 10 minute at room temperature. Crosslinked cells were lysed, sonicated and chromatin was collected for IP. 2-5 ug of antibody was used for each IP. IPed chromatin was captured by Protein A beads. After washing, bound complexes were eluted and crosslinking was reversed. DNA was extracted using phenol-chloroform and purified by ethanol precipitation.