Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

ESC cells

cell type

ESC cells

antibody

Anti Pol II antibody (QED Bioscience)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, cells were harvested and crosslinked in suspension with 1% formaldehyde for 10 minute at room temperature. Crosslinked cells were lysed, sonicated and chromatin was collected for IP. 2-5 ug of antibody was used for each IP. IPed chromatin was captured by Protein A beads. After washing, bound complexes were eluted and crosslinking was reversed. DNA was extracted using phenol-chloroform and purified by ethanol precipitation.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

61957164

Reads aligned (%)

98.4

Duplicates removed (%)

40.3

Number of peaks

29936 (qval < 1E-05)

mm9

Number of total reads

61957164

Reads aligned (%)

98.2

Duplicates removed (%)

40.4

Number of peaks

29831 (qval < 1E-05)