GSM2520004: YXY1077 RAP IP Ino80 TBPAA; Saccharomyces cerevisiae; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
INO80
Cell type
Cell type Class
Yeast strain
Cell type
MJE7
NA
NA
Attributes by original data submitter
Sample
source_name
bar1∆, TBP-FRB, Ino80-13Myc
cell type
yeast cells
strain
MJE7 Background
antibody
Anti Myc antibody from Abcam (ab32)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Samples were lysed with glass beads in lysis buffer, chromatin was collected by centrifugation, resuspended in lysis buffer and subjected to sonification. The supernatant from sonicated lysates were precleared with Protein A beads and ChIP was performed as described using commercial antibodies Libraries were prepared with a KAPA LTP kit following the manufacturer's protocol