Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Cell line

Cell type

S2

Source

Oregon R

Developmental Stage

late embryonic stage

Sample

source_name

Endogenous BRM Input

cell line

S2 cells

chip antibody

Monoclonal against Drosophila BRM (non-commercial)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Input DNA was isolated with the ChIP cleanup and concentrator Kit (Zymo Research) Input DNA and immunoprecipitated DNA samples were used for Rubicon Thruplex-FD library preparation and sequenced in a Illumina HiSeq2500 instrument

Sequencing Platform

instrument_model

Illumina HiSeq 2500

dm6

Number of total reads

22211482

Reads aligned (%)

97.0

Duplicates removed (%)

15.9

Number of peaks

3505 (qval < 1E-05)

dm3

Number of total reads

22211482

Reads aligned (%)

97.4

Duplicates removed (%)

13.4

Number of peaks

3297 (qval < 1E-05)