Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Epidermis

Cell type

BJ

Primary Tissue

Skin

Tissue Diagnosis

Normal

Sample

source_name

Primary fibroblast transformed with hTERT and SV40 early region

cell line

BJ-HELT

transfection

siTRF2

cell type

human foreskin fibroblast

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and yH2AX-DNA complexes were isolated with antibody. Libraries were prepared according to standards Illumina's instructions. yH2AX antibody from Abcam (ab2893), rabbit polyclonal

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

43958664

Reads aligned (%)

99.2

Duplicates removed (%)

2.8

Number of peaks

1431 (qval < 1E-05)

hg19

Number of total reads

43958664

Reads aligned (%)

98.3

Duplicates removed (%)

4.4

Number of peaks

1345 (qval < 1E-05)