Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Neural

Cell type

Glioblastoma

MeSH Description

A malignant form of astrocytoma histologically characterized by pleomorphism of cells, nuclear atypia, microhemorrhage, and necrosis. They may arise in any region of the central nervous system, with a predilection for the cerebral hemispheres, basal ganglia, and commissural pathways. Clinical presentation most frequently occurs in the fifth or sixth decade of life with focal neurologic signs or seizures.

Sample

source_name

human glioblastoma sample

cell type

glioblastoma, brain tumor initiating cells

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

1 million cells were crosslinked in 1% formaldehyde for 10 minutes, lysed, sonicated to ~500bp, and immunoprecipated using H3K27ac antibody. ChIP DNA was purified using the Qiagen PCR purification kit. Libraries were prepared according to manufacturers instructions using the Rubicon Thruplex DNA-seq kit

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

17173431

Reads aligned (%)

88.4

Duplicates removed (%)

5.2

Number of peaks

21795 (qval < 1E-05)

hg19

Number of total reads

17173431

Reads aligned (%)

88.0

Duplicates removed (%)

5.3

Number of peaks

21585 (qval < 1E-05)