GSM2496946: Pol II CHIP-seq 30min AcD1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Blood
Cell type
CD4 CD8 double negative cells
NA
NA
Attributes by original data submitter
Sample
source_name
in-vitro DN2 cells
strain
C57BL/6
genotype
WT
chip antibody
RNA pol-II (8WG16), ab817, GR299078-2
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lived DN2 cells were selected on day10. Cells were cultured as control without OP9-DL1 n aMEM 20% FBS, IL7, FLT3-L, with 10 ug/ml of actinomycin-d . Cells were harvested at 30 min, immediately spun down, and resuspended in PBS. Cells were fixed with 1% formaldehyde. Formaldehyde was quenched with 0.2 M glycine for 10 min. Fixed cells were stored at -80 degrees until use. Libraries were prepared with NEBNext primer set. Fragment size was selected with AMPure beads (Beckman Coulter).