Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Liver
Cell type
Liver
MeSH Description
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Attributes by original data submitter

Sample

source_name
Liver
strain
C57BL/6
experiment
ChIP-Seq

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq was performed with the ‘MicroPlex Library Preparation’ kit, from Diagenode 10 ng of immunoprecipitated material was used for sequencing library preparation. Total input was also prepared for sequencing at each time point and replicate. Paired-end sequencing libraries were prepared with the ‘MicroPlex Library Preparation’ kit, from Diagenode, following the instructions of the manufacturer (Diagenode, catalog no C05010011). No size selection was performed and the chromatin was amplified with 14 cycles of PCR. 100 nucleotides at the fragment ends were sequenced with paired-end sequencing technology from HiSeq 2100 (Illumina).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
216663637
Reads aligned (%)
0.0
Duplicates removed (%)
18.1
Number of peaks
642 (qval < 1E-05)

mm9

Number of total reads
216663637
Reads aligned (%)
0.0
Duplicates removed (%)
18.5
Number of peaks
662 (qval < 1E-05)

Base call quality data from DBCLS SRA