Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Pluripotent stem cell

Cell type

P19

Tissue

Embryo

Disease

Teratocarcinoma; Embryonal Carcinoma

Sample

source_name

P19 cells

cell line

P19

cell type

embryonic carcinoma cells

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with the DPPA4 antibody (R&D systems AF3730). Libraries were prepared with the Nextera library prep kit

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

21443378

Reads aligned (%)

94.9

Duplicates removed (%)

17.7

Number of peaks

24009 (qval < 1E-05)

mm9

Number of total reads

21443378

Reads aligned (%)

94.6

Duplicates removed (%)

17.7

Number of peaks

23892 (qval < 1E-05)