GSM2492395: shSmyd5 H3K9me3 CC (GA6717); Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K9me3
Cell type
Cell type Class
Others
Cell type
Cancer cells
NA
NA
Attributes by original data submitter
Sample
source_name
shSmyd5_CC
genotype/variation
Smyd5 depletion
cell type
cancer cells (CC)
chip antibody
Anti-H3K9me3 (Abcam; ab8898)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with formaldehyde and subsequently sonicated. Sonicated cell extracts were used for ChIP assays. ChIP-enriched DNA was end-repaired using the End-It DNA End-Repair kit (Epicentre), followed by addition of a single A nucleotide, and ligation of PE adapters (Illumina) or custom indexed adapters. PCR was performed using Phusion High Fidelity PCR master mix