Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Mast Cells

MeSH Description

Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.

Sample

source_name

MC9 shRad21

chip antibody

H3K27ac (Abcam ab4729 Lot: GR243994-1)

cell line

MC9 shRad21 Mast

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

See ChIP-Endo Protocol.pdf

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

12343893

Reads aligned (%)

91.7

Duplicates removed (%)

2.9

Number of peaks

473 (qval < 1E-05)

mm9

Number of total reads

12343893

Reads aligned (%)

91.6

Duplicates removed (%)

3.0

Number of peaks

461 (qval < 1E-05)