Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
YAP1

Cell type

Cell type Class
Cardiovascular
Cell type
HBMEC
Tissue
blood vessel
Lineage
mesoderm
Description
brain microvascular endothelial cells

Attributes by original data submitter

Sample

source_name
HBMECs
treatment
VEGF treatment (6h)
antibody
YAP/TAZ (D24E4) Rabbit mAb (Cell signalling)
beads
Protein A/G magnetic beads (Pierce)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were then fixed with 1% formaldehyde (sigma) in medium for 10min at room temperature, and lysed in 1%SDS lysis buffer. Chromatin was sheared to approximately 200bp fragments by using Covaris S220X. Libraries were generated by ThruPLEX DNA-seq Kit (Rubicon).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
23145262
Reads aligned (%)
39.4
Duplicates removed (%)
54.7
Number of peaks
758 (qval < 1E-05)

hg38

Number of total reads
23145262
Reads aligned (%)
41.3
Duplicates removed (%)
52.9
Number of peaks
577 (qval < 1E-05)

Base call quality data from DBCLS SRA