Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Unclassified
Cell type
Unclassified
NA
NA

Attributes by original data submitter

Sample

source_name
VEGF_minus_48h_H3K27me3
culture condition
without VEGF
time
48 hours after without VEGF
chip antibody
H3K27me3 (#07-449, Millipore, Billerica, MA, USA)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, sonicated nuclear fraction was incubated with each antibody and histone-DNA were isolated. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
39297057
Reads aligned (%)
97.2
Duplicates removed (%)
31.4
Number of peaks
603 (qval < 1E-05)

mm9

Number of total reads
39297057
Reads aligned (%)
97.0
Duplicates removed (%)
31.4
Number of peaks
719 (qval < 1E-05)

Base call quality data from DBCLS SRA