Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Unclassified

Cell type

Unclassified

NA

NA

Sample

source_name

VEGF_plus_6h_H3K4me3

culture condition

with VEGF

time

6 hours after VEGF stimulation

chip antibody

H3K4me3 (non-commercial, gifted by Dr. Hiroshi Kimura, Tokyo Institute Technology, Japan)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, sonicated nuclear fraction was incubated with each antibody and histone-DNA were isolated. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

mm10

Number of total reads

24133517

Reads aligned (%)

88.7

Duplicates removed (%)

38.1

Number of peaks

4189 (qval < 1E-05)

mm9

Number of total reads

24133517

Reads aligned (%)

88.6

Duplicates removed (%)

38.3

Number of peaks

4207 (qval < 1E-05)