Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase III

Cell type

Cell type Class

Pluripotent stem cell

Cell type

NT2-D1

Tissue

testis

Lineage

inner cell mass

Description

malignant pluripotent embryonal carcinoma (NTera-2), "The NTERA-2 cl.D1 cell line is a pluripotent human testicular embryonal carcinoma cell line derived by cloning the NTERA-2 cell line." - ATCC. (PMID: 6694356)

Sample

source_name

Embryonal carcinoma stem cells

cell type

NT2D1

passages

p37

genotype

46 XY +complex

chip antibody

anti-POLR3G (SZ3070, Prof. Nouria Hernandez)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Qiagen all prep for smallRNA (S7-13) and mRNA (S1-6), ChIP-DNA (S14-18): Qiagen MinElute PCR Purification Kit smallRNA-seq (S7-13): Illumina TruSeq smallRNA, mRNA-seq (S1-6): Illumina TruSeq mRNA, ChIP-seq (S14-18): Diagenode Microplex Library Preparation kit v2

Sequencing Platform

instrument_model

Illumina HiSeq 3000

hg38

Number of total reads

27632141

Reads aligned (%)

98.4

Duplicates removed (%)

12.2

Number of peaks

1126 (qval < 1E-05)

hg19

Number of total reads

27632141

Reads aligned (%)

97.7

Duplicates removed (%)

13.6

Number of peaks

1218 (qval < 1E-05)