GSM2478333: monoclonal Flag ChIP-Seq; 8567cells; 0C, minus tetracycline; Biological replicate 2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Epitope tags
Cell type
Cell type Class
Breast
Cell type
3134
Tissue
Mammary
Lineage
cellLine
Description
Mammary
Attributes by original data submitter
Sample
source_name
3134 adenocarcinoma cells
antibody
anti-Flag (Sigma anti-Flag F1804)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde, lysed and sonicated to small DNA fragments. IPs were performed with anti-BRG1 (1 μg, EPNCIR11A, made in collaboration with Epitomics, Burlingame, CA), or anti-Flag (4ug Sigma anti-Flag F1804) antibody. ChIP-Seq libraries were made by minimal PCR amplification cycles using the Illumina adaptor and PCR primers. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.