Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Muscle
Cell type
Myotube
NA
NA

Attributes by original data submitter

Sample

source_name
in vitro differentiated myotubes
condition
Chromatin belonging to 2 days in vitro differentiated myotubes
library strategy
ChIP-Seq (ChIRP-Seq)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysed using Trizol and RNA was extracted using qiagen RNA extraction kit. TruSeq Stranded mRNA Library Prep Kit The crosslinked chromatin was sonicated and the Charme RNA, together with the co-precipitated DNA, was purified. NuGEN Ovation Ultralow Library System v2

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
78269698
Reads aligned (%)
94.2
Duplicates removed (%)
36.6
Number of peaks
1073 (qval < 1E-05)

mm9

Number of total reads
78269698
Reads aligned (%)
93.8
Duplicates removed (%)
36.4
Number of peaks
1250 (qval < 1E-05)

Base call quality data from DBCLS SRA